In the frame of recently funded EU-H2020-MSC-ITN project MarPipe (Improving the flow in the pipeline of the next generation of marine biodiscovery scientists), GEOMAR Helmholtz Centre for Ocean Research Kiel is offering a Early Stage Researcher (ESR) - PhD position: Enhanced chemical diversity of antibiotic molecules from extremophilic microorganisms monitored by metabolomics and chemical imaging starting on 1. April 2017.
MarPipe will study the further development of antimicrobial and anticancer lead compounds originating from a previous EU project (PharmaSea), and will also explore the bioactivity of deep-sea samples collected in the sub-Antarctic.
The main aim of the PhD project (ESR5) at GEOMAR will be the discovery of new molecules from marine microbes from these extreme environments. The project will involve innovative cultivation techniques of the extremophilic microorganisms (by OSMAC and co-cultivation), their extraction, bioactivity testing and metabolome profiling (the latter by LC-MS and 1H NMR), chemical imaging of microbial metabolites in mono- and co-cultures (by DESI-Imaging Mass Spectrometry), purification of the bioactive metabolites (by HPLC) and structure elucidation (by NMR, LC-MS and other spectroscopic techniques). The compounds will then be evaluated for bioactivity against a large panel of assays.